Title A Novel Core Fucose - specific Lectin from the Mushroom
نویسندگان
چکیده
Core fucose-specific lectin from Pholiota squarrosa 1 A novel core fucose-specific lectin from the mushroom Pholiota squarrosa* Background: Fuc-α1-6 oligosaccharide has a variety of biological functions. Results: Purification of a novel Fucα 1-6-specific lectin from the mushroom Pholiota squarrosa. Conclusion: The lectin binds only to core α1-6 fucosylated N-glycans and not to the other types of fucosylated oligosaccharides. Significance: The lectin will be a promising tool for analyzing the biological functions of α1-6 fucosylation.
منابع مشابه
A novel core fucose-specific lectin from the mushroom Pholiota squarrosa.
Fucα1-6 oligosaccharide has a variety of biological functions and serves as a biomarker for hepatocellular carcinoma because of the elevated presence of fucosylated α-fetoprotein (AFP) in this type of cancer. In this study we purified a novel Fucα1-6-specific lectin from the mushroom Pholiota squarrosa by ion-exchange chromatography and affinity chromatography on thyroglobulin-agarose. The puri...
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A novel fucose-binding lectin (SL2-1) from the bacterium Streptomyces rapamycinicus was identified by analysis of metagenomic DNA sequences. SL2-1 belongs to a new group of bacterial fucose-specific lectins that have no similarity to known bacterial fucose-binding proteins, but are related to certain eukaryotic fucose-binding lectins. The 17 kDa protein was expressed recombinantly in E. coli an...
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Using an aMnity adsorbent prepared from L-fucose and starch, a lectin was isolated from fruit bedies of an ascomycete mushroom, IVdlelastiza ch teri. The lectin was found to cross-react with antiserum against Aleuria aurantia lectin (AAL), that had been obtained £ rom another ascomycete mushroom. The N-termina] amino acid sequence was analyzed, and among 20 residues 12 were the same as AAL. The...
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Background and purpose : The role of glycoconjugate terminal sugars on development of fetal tissues as inducing factors has been distinguished. Lectinhistochemistry tracing of α-L- fucose terminal sugar, defining its effective time and elucidating its specific role in development of the mouse kidney were among the main objectives of this study. Materials and methods : Fetuses of Balb/c pre...
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The alpha1,6-fucosyl residue (core fucose) of glycoproteins is widely distributed in mammalian tissues and is altered under pathological conditions. A probe that specifically detects core fucose is important for understanding the role of this oligosaccharide structure. Aleuria aurantia lectin (AAL) and Lens culimaris agglutinin-A (LCA) have been often used as carbohydrate probes for core fucose...
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